Transcriptome analysis of Aofus3 in nematode-trapping fungus Arthrobotrys oligospora. Transcriptome analysis of Aofus3 in nematode-trapping fungus Arthrobotrys oligospora

Transcriptomic analysis revealed that differentially expressed genes in the absence of Aofus3 were involved in cell fusion, repair, and cellcommunication during trap formation. Overall design: WT and ΔAofus3 strains grown on cellophane-overlaid PDA plates at 28°C for 5 days. Approximately 600 nematodes were then added to each plate for 0, 12, 24, and 36 h of induction, three replicates were used for each sample. After collecting the samples, RNA was extracted with the AxyPrep Multisource RNA Miniprep Kit (Axygen, Jiangsu, China), and then sent to Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China) to construct the transcriptome of WT and ΔAofus3 strains. The final cDNA library was sequenced on an Illumina Novaseq 6000 platform.

转录组分析结果显示，缺失Aofus3后的差异表达基因，在捕器形成过程中参与了细胞融合、修复及细胞通讯过程。实验设计概述：将野生型（Wild Type, WT）与ΔAofus3菌株接种于覆盖玻璃纸的马铃薯葡萄糖琼脂（Potato Dextrose Agar, PDA）平板，于28℃培养5天。随后向每块平板加入约600条线虫，分别进行0、12、24、36小时的诱导处理，每个样本设置3次生物学重复。样本收集完成后，使用AxyPrep多源RNA小量制备试剂盒（Axygen，中国江苏）提取RNA，随后送至上海美吉生物医药科技有限公司（Majorbio Bio-Pharm Technology Co., Ltd.，中国上海）构建野生型与ΔAofus3菌株的转录组文库。最终构建的cDNA文库在Illumina Novaseq 6000测序平台上完成测序。