The role of altered lipid composition and distribution in liver fibrosis revealed by multimodal nonlinear optical microscopy

To reveal the involved gene regulatory pathways, we carried out RNA-seq transcriptomics analysis on the cells treated with or without PA, treated with PA+OA and PA+BMS. Overall design: con: HepaRG without treated for 4h. PA: HepaRG were treated with 400µM deuterium-labeled palmitate acid (PA) for 4h. PA+OA: HepaRG were treated with 400µM PA and 200µM oleic acid (OA) for 4h PA+BMS: HepaRG were treated with 50µM BMS-309403 (BMS) for 24 h during concomitant incubation with 400µM PA for 4 h.

为揭示所涉及的基因调控通路，我们对经PA处理与未处理的HepaRG细胞、以及经PA+OA和PA+BMS处理的HepaRG细胞开展了RNA测序（RNA-seq）转录组学分析。实验总体设计如下：对照组（con）：未经任何处理的HepaRG细胞孵育4小时。PA组：采用400μM氘标记棕榈酸（PA）处理HepaRG细胞4小时。PA+OA组：采用400μM PA与200μM油酸（OA）共同处理HepaRG细胞4小时。PA+BMS组：在与400μM PA共同孵育4小时的过程中，用50μM BMS-309403（BMS）处理细胞24小时。