RNA-Seq analysis to study the transcriptomic profiles of mice after 4-month wheel running exercise

To identify the mechanism underlying AdipoR1-mediated beneficial effect of exercise, we used RNA-Seq analysis to study the transcriptomic profiles of mice after 4-month wheel running exercise. Differential expression analysis was performed by comparing exercised mice to control mice, and exercised mice subjected to AdipoR1 shRNA versus exercised mice. The transcriptional profiles of gastrocnemius muscle of exercised mice, control mice, and exercised mice subjected to AdipoR1 shRNA were generated by deep sequence, in quadruplicate, using Illumina Novaseq™.

为阐明AdipoR1（Adiponectin Receptor 1）介导运动益处的潜在分子机制，本研究通过RNA测序（RNA-Seq）分析，探究经4个月跑轮运动的小鼠转录组谱特征。实验通过两组对比完成差异表达分析：其一为运动小鼠与对照组小鼠，其二为接受AdipoR1短发夹RNA（short hairpin RNA, shRNA）处理的运动小鼠与普通运动小鼠。本研究采用Illumina NovaSeq™平台，对上述三组小鼠的腓肠肌转录组进行深度测序，每组均设置4次生物学重复。