Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of Pericarps from Wild-type and Mutant Fruits.. Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of Pericarps from Wild-type and Mutant Fruits.

Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to analyze the difference of gene expression between wild-type and mutant during tomato fruit ripening progress. Overall design: Fruit mRNA profiles of mature green (35) and breaker (38) stages in both wild-type (A) and mutant (cr) were generated by deep sequencing, in triplicate, using Illumina Novoseq 6000.

下一代测序（Next-generation sequencing，NGS）技术已然革新了细胞通路的系统级分析研究范式。本研究旨在解析番茄果实成熟进程中野生型与突变体样本间的基因表达差异。实验设计概述：针对野生型（A）与突变体（cr）的番茄果实，分别采集绿熟期（标注为35）与转色破色期（标注为38）的样本，对其mRNA转录组进行深度测序，每组设置三次重复，测序采用Illumina Novoseq 6000平台。