Developing Biomarkers Incorporating High Throughput RNA, DNA, Small RNA Sequencing and Protein Expression in Inflammatory Bowel Disease Using Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue Samples

This study included pediatric patients with suspected inflammatory bowel disease (IBD) who underwent endoscopy between 2008 and 2012. This included 101 colonic tissue samples and 100 ileal tissue samples. Biopsies were obtained at the time of diagnosis, and patients were followed for a mean of 6 years to record clinical characteristics. These included baseline characteristics, such as age of diagnosis and initial Montreal classification, as well as outcomes, such as stricturing or fistulizing complications, disease remission, or progression to surgery.Genes with significant results in the non-IBD vs CD comparison in colonic tissue are listed in the "Links to Related Genes" section of this page.]]>
Inclusion Criteria:Pediatric patients (<18 years old) with suspected IBD undergoing endoscopy who were found either to have uncomplicated, treatment-naive Crohn's disease or no IBD were included. Exclusion Criteria:Patients with complicated Crohn's disease (including strictures or fistulas) or ulcerative colitis were excluded.]]>

本研究纳入2008年至2012年间接受内镜检查的疑似炎症性肠病（Inflammatory Bowel Disease, IBD）儿科患者，共收集101份结肠组织样本与100份回肠组织样本。所有活检样本均采集于确诊当日，研究团队对患者平均随访6年以记录其临床特征，包括确诊年龄、初始蒙特利尔分型等基线特征，以及狭窄型或瘘管型并发症、疾病缓解、手术进展等转归结局。在结肠组织的非IBD与克罗恩病（Crohn's Disease, CD）对比分析中获得显著统计学结果的基因，已列于本页面的"相关基因链接"板块。

纳入标准：纳入年龄<18岁的疑似IBD儿科患者，经内镜检查后确诊为未合并并发症且未接受过治疗的克罗恩病，或确诊无IBD者。
排除标准：排除合并复杂克罗恩病（包括狭窄或瘘管）或溃疡性结肠炎的患者。