Targeting macrophage M1 polarization suppression through PCAF inhibition alleviates autoimmune arthritis via synergistic NF-?B and H3K9Ac blockade

M1 macrophages are considered to contribute to autoimmune arthritis such as rheumatoid arthritis, spondyloarthritis. However, the exact role of M1 macrophage during inflammatory joint disease remain unclear. To explore the molecular mechanisms underlying M1 macrophage in autoimmune arthritis, we performed the whole transcriptome sequencing of 3 repeats of M0, 3 repeats of M1 without 20 µM Garcinol and 3 repeats of M1 with 20 µM Garcinol. Overall design: Total 9 samples were analyzed. The whole transcriptome sequencing of 3 repeats of M0, 3 repeats of M1 without 20 µM Garcinol and 3 repeats of M1 with 20 µM Garcinol was performed.

M1型巨噬细胞（M1 macrophage）被认为在类风湿关节炎、脊柱关节炎等自身免疫性关节炎的发生发展中发挥作用。然而，M1型巨噬细胞在炎症性关节疾病中的确切作用仍未明确。为探究M1型巨噬细胞在自身免疫性关节炎中的潜在分子机制，我们对3个生物学重复的M0巨噬细胞（M0 macrophage）样本、3个生物学重复的未经20 μM藤黄菌素（Garcinol）处理的M1型巨噬细胞样本，以及3个生物学重复的经20 μM藤黄菌素处理的M1型巨噬细胞样本开展了全转录组测序。整体实验设计：本研究共分析9份样本，具体为对3个生物学重复的M0巨噬细胞样本、3个生物学重复的未添加20 μM藤黄菌素的M1型巨噬细胞样本，以及3个生物学重复的添加20 μM藤黄菌素的M1型巨噬细胞样本实施全转录组测序。