scRNA of lichen planus

An early and sustained clinical response was seen, with 83.3% of patients responsive at week 16. Our molecular data identified a unique, oligoclonal IFN-γ, CD8+, CXCL13+ cytotoxic T-cell population in LP skin and demonstrated a rapid decrease in IFN signature within 2 weeks of treatment, most prominently in the basal layer of the epidermis. We conducted scRNA for skin and pbmcs in LP patients and normal individuals

本研究观察到早期且持续的临床应答，83.3%的患者在第16周时呈现阳性应答。我们的分子数据在扁平苔藓（Lichen Planus, LP）患者的皮肤组织中鉴定出一类独特的寡克隆干扰素γ（IFN-γ）阳性、CD8阳性、CXCL13阳性的细胞毒性T细胞群，并证实治疗后2周内干扰素特征（IFN signature）快速下调，该变化在表皮基底层最为显著。我们对LP患者与健康个体的皮肤组织及外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）开展了单细胞RNA测序（single-cell RNA sequencing, scRNA）。