Bacterial abundance in the eastern Mediteranean Sea in August and September 2008 during SES_GR2

The SES_GR2_MICROBIAL PARAMETERS dataset is based on samples collected in the framework of the project SESAME, in the Ionian, Libyan and Aegean Sea during August-September 2008. The objectives were to measure the standing stocks and calculate the production of the microbial compartment of the food web, describe the vertical distribution pattern and characterize its structure and function through the water column. Subsamples for virus, heterotrophic bacteria and cyanobacteria (Synechococcus spp. and Prochlorococcus spp.) counting were analyzed using a FACSCalibur (Becton Dickinson) flow cytometer equipped with a standard laser (488 nm) and filter set and using deionized water as sheath fluid. Fluorescent beads with a diameter of 0.97 μm (Polysciences) were added to each sample as an internal standard, and all parameters were normalized to the beads and expressed as relative units. SYBRGreen I stain (Molecular Probe) was used to stain viral and heterotrophic bacterial DNA. Viruses were counted according to (Brussaard 1984). In order to avoid bulk consentrations of viruses samples we dilluted to Tris-EDTA (pH=8,0) buffer to a final sollution of 1/5 to 1/100. Total abundance and nucleid content classes were calculated using the Paint-A-Gate software (Becton Dickinson).

SES_GR2_MICROBIAL PARAMETERS数据集基于SESAME项目框架下采集的样本，于2008年8月至9月在爱奥尼亚海、利比亚海及爱琴海获取。本数据集的研究目标为：测定食物网微生物组分的现存量并计算其生产力，描述其垂直分布格局，并表征整个水柱中该组分的结构与功能。用于病毒、异养细菌及蓝细菌（聚球藻属Synechococcus spp.和原绿球藻属Prochlorococcus spp.）计数的子样本，采用配备标准激光（488 nm）及滤光片组的FACSCalibur（Becton Dickinson）流式细胞仪进行分析，鞘液使用去离子水。向每个样本中加入直径为0.97 μm的荧光微球（Polysciences）作为内标，所有参数均以该微球为基准进行归一化，并以相对单位表示。使用SYBRGreen I染色液（Molecular Probe）对病毒及异养细菌的DNA进行染色。病毒计数参考Brussaard 1984的方法。为避免病毒样本浓度过高，采用Tris-EDTA（pH=8.0）缓冲液将样本稀释至终比例为原样本的1/5至1/100。使用Paint-A-Gate软件（Becton Dickinson）计算总丰度及核酸含量类别。