SH1426226.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE为界定、识别、交流以及处理基于DNA的物种假说（Species Hypotheses，以下简称SH）提供了统一的标准化路径。国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，均通过应用一组动态距离阈值（0.5%~3.0%）聚类至近似物种级别的单元。所有物种假说均被赋予以数字对象标识符（Digital Object Identifier，DOI）为形式的唯一且稳定的标识名称，其分类学与生态学注释信息将通过分布式网络第三方注释工作完成审核验证。各物种假说会尽可能结合该假说下所有序列的鉴定结果，关联至对应的分类单元名称及其所属分类层级（如门、纲、目等）。每条物种假说均会选取一条经自动或人工指定的序列作为其代表序列。上述代表序列已在https://unite.ut.ee/repository.php 公开发布，可供科研群体用于本地序列相似性搜索、下一代测序数据分析流程等各类科研场景。随着公共真菌ITS序列数据体量的持续增长，该系统与配套数据集将自动完成更新迭代。