Total phenolic, flavonoid, alkaloid and iridoid content and preventive effect of Lider-7-tang on lipopolysaccharide-induced acute lung injury in rats

Lider-7-tang, a medicine used for the treatment of respiratory diseases especially pneumonia and fever in Mongolian Traditional Medicine, was selected for this phytochemical and pharmacological study. The objectives of the study were to determine total biological active substances and analyze the effects of Lider-7-tang treatment in rats with acute lung injury (ALI). Quantitative determination of the total active constituents (phenolic, flavonoid, iridoid and alkaloid) of the methanol extract of Lider-7-tang was performed using Folin-Ciocalteu reagent, aluminum chloride reagent, Trim-Hill reagent, and Bromocresol green reagent, respectively. A total of fifty 8–10-week-old male Wistar rats (200–240 g) were randomized into three groups: control group, lipopolysaccharide (LPS) group (7.5 mg/kg) and LPS+Lider-7 group (90 mg/kg Lider-7-tang before LPS administration). The total content of alkaloids was 0.2±0.043%, total phenols 7.8±0.67%, flavonoids 3.12±0.206%, and iridoids 0.308±0.0095%. This study also evaluated the effects of Lider-7 on levels of inflammatory mediators by observing histopathological features associated with LPS-induced ALI. The rats pretreated with Lider-7 had significantly lower levels of IL-6 (at 3 and 6 h), and TNF-α (at 3, 6, 9, and 12 h). The current study showed that Lider-7 exerted a preventive effect against LPS-induced ALI, which appeared to be mediated by inhibiting the release of pro-inflammatory cytokines.

本植物化学与药理学研究选取了蒙药中用于治疗呼吸系统疾病（尤其肺炎与发热）的利德七汤（Lider-7-tang）作为研究对象。本研究旨在测定其总生物活性物质含量，并分析利德七汤对急性肺损伤（ALI）模型大鼠的干预效果。采用福林-酚试剂（Folin-Ciocalteu reagent）、氯化铝试剂、Trim-Hill试剂、溴甲酚绿试剂，分别对利德七汤甲醇提取物中的总活性成分——酚类、黄酮类、环烯醚萜类及生物碱类物质进行定量测定。共计50只8~10周龄、体重200~240g的雄性Wistar大鼠，被随机分为3组：空白对照组、脂多糖（LPS）模型组（造模剂量7.5mg/kg）以及LPS+利德七汤干预组（LPS造模前给予90mg/kg利德七汤）。经测定，利德七汤甲醇提取物中生物碱类总含量为0.2±0.043%，总酚类为7.8±0.67%，黄酮类为3.12±0.206%，环烯醚萜类为0.308±0.0095%。本研究同时通过观察脂多糖诱导急性肺损伤大鼠的组织病理学特征，评估利德七汤对炎症介质水平的调控作用。预先给予利德七汤的干预组大鼠，其IL-6水平在造模后3、6小时显著降低，肿瘤坏死因子α（TNF-α）水平在造模后3、6、9、12小时均显著低于模型组。本研究结果表明，利德七汤对脂多糖诱导的急性肺损伤具有预防保护作用，其作用机制可能与抑制促炎细胞因子的释放有关。