Table_3_Genome-wide identification, characterization, and functional analysis of lncRNAs in Hevea brasiliensis.xlsx

Natural rubber (NR) is an essential industrial raw material widely used in our life. Hevea brasiliensis (Reyan7-33-97) is an economic plant producing natural rubber. Long non-coding RNAs (lncRNAs) are emerging as crucial regulators in numerous biological processes while the characterization and analysis of lncRNAs in Hevea brasiliensis are still largely unrevealed. We integrated the transcriptome datasets from multiple tissues to identify rubber lncRNAs. As a result, 12,029 lncRNAs were found and characterized with notably distinctive features such as longer exon, lower expression levels and GC content, and more tissue specificity in comparison with mRNAs. We discovered thousands of tissue-specific lncRNAs in rubber root, latex, bark, leaf, flower, and seed tissues. The functional enrichment result reveals that tissue-specific lncRNAs are potentially referred to particular functions of tissues, while the non-tissue specific is related to the translation and metabolic processes. In the present study, a comprehensive lncRNA dataset was identified and its functional profile in Hevea brasiliensis was explored, which provides an annotation resource and important clues to understand the biological functions of lncRNAs in Hevea brasiliensis.

天然橡胶（Natural Rubber，NR）是一类至关重要的工业原材料，广泛应用于日常生活中。巴西橡胶树（Hevea brasiliensis，品种Reyan7-33-97）是生产天然橡胶的经济作物。长链非编码RNA（Long non-coding RNAs，lncRNAs）已被证实是众多生物过程中的关键调控因子，但目前针对巴西橡胶树中长链非编码RNA的鉴定与分析研究仍尚未得到充分揭示。本研究整合多组织转录组数据集，以鉴定橡胶树中的长链非编码RNA。最终共鉴定并注释了12029条长链非编码RNA，相较于信使RNA（messenger RNA，mRNA），这些长链非编码RNA具有显著独特的特征：外显子更长、表达水平与GC含量更低，且组织特异性更强。研究人员在橡胶树的根、胶乳、树皮、叶片、花与种子组织中，发现了数千条组织特异性长链非编码RNA。功能富集分析结果显示，组织特异性长链非编码RNA可能参与对应组织的特定功能调控，而非组织特异性长链非编码RNA则主要与翻译及代谢过程相关。本研究成功鉴定得到一套完整的巴西橡胶树长链非编码RNA数据集，并对其在巴西橡胶树中的功能特征进行了探究，该数据集可为解析巴西橡胶树中长链非编码RNA的生物学功能提供重要的注释资源与研究线索。