Milbemycins: more than efflux inhibitors for fungal pathogens

This study investigated the effect of milbemycons as efflux inhibitors and antifungal agents. Milbemycin oxims can inhibit growth of Candida glabrata and C. albicans. The effect of milbemycins on transcriptomes was inbvestigated. Gene expression was measured in C. glabrata milbemycin-treated cells (1h) and compared to untreated cells in the incubation mediun RPMI. The one-color system was used. Four biological replicates were used for each condition. Gene expression was measured in C. albicans milbemycin-treated cells (1h) and compared to untreated cells in the incubation mediun RPMI. The one-color system was used. Three biological replicates were used for each condition.

本研究探究了米尔贝霉素类（milbemycins）作为外排泵抑制剂（efflux inhibitors）与抗真菌剂（antifungal agents）的作用效果。米尔贝霉素肟类（milbemycin oxims）可抑制光滑念珠菌（Candida glabrata）与白色念珠菌（Candida albicans）的生长。本研究分析了米尔贝霉素类对转录组（transcriptomes）的影响：以RPMI培养基为孵育介质，对经米尔贝霉素处理1小时的光滑念珠菌细胞进行基因表达检测，并与未处理的对照细胞进行比较，实验采用单通道系统（one-color system），每组设置4个生物学重复（biological replicates）。针对白色念珠菌的实验如下：以RPMI培养基为孵育介质，检测经米尔贝霉素处理1小时的白色念珠菌细胞的基因表达水平，并与未处理的对照细胞比较，同样采用单通道系统，每组设置3个生物学重复。