HNF4a regulates hepatic peroxisomal biogenesis and fatty acid elongation during the thermogenic response

We observed gene expression changes in livers of cold exposed mice along with changes in global genome occupancy for the transcription factor HNF4a, RNA polymerase II and the histone marker H3K27Ac, suggesting a hepatic transcriptional response to cold xposure. Performed RNA-seq for gene expression changes on livers of cold exposed mice during a 24 hour time course (hours 0,6,12,24). Perforemed ChIP-seq for HNF4a, Rpb1-CTD, H3K27Ac on livers from room temperture and cold exposed (6 hours) mice.

本研究检测了冷暴露小鼠肝脏中的基因表达变化，同时分析了转录因子肝细胞核因子4α（HNF4a）、RNA聚合酶II（RNA polymerase II）以及组蛋白标记H3K27Ac（H3K27Ac）的全基因组结合占用谱变化，结果提示小鼠肝脏存在针对冷暴露的转录应答反应。我们针对24小时时间进程（0、6、12、24小时）内的冷暴露小鼠肝脏样本开展了RNA测序（RNA-seq），以检测其基因表达变化。同时对室温饲养及冷暴露6小时的小鼠肝脏样本，开展了针对HNF4a、Rpb1-CTD及H3K27Ac的染色质免疫共沉淀测序（ChIP-seq）实验。