A genome-wide RNAi screen for T. brucei MMS damage response factors. MMS effect on RITseq in T.brucei

To identify genome maintenance pathways active in a divergent eukaryote and important parasite, Trypanosoma brucei, we performed RNAi screens to search for genes important for survival following exposure to the alkylating agent methyl methanesulphonate. Amongst a cohort of broadly conserved and, therefore, early evolved T. brucei repair pathways, we reveal novel Trypanosoma- or kinetoplastid-specific repair-associated activities.

为鉴定在演化歧异的重要寄生真核生物布氏锥虫（Trypanosoma brucei）中活跃的基因组维持通路，我们开展了RNA干扰（RNAi）筛选，以寻找经烷化剂甲基磺酸甲酯处理后对虫体存活不可或缺的基因。在一组广泛保守、因而属于早期演化的布氏锥虫修复通路中，我们揭示了全新的锥虫特异性或动质体（kinetoplastid）特异性修复相关活性。