Multi-tissue expression profiling of TALLYHO/Jng and C57BL/6J Mice under high-fat and chow diets

In order to identify gene expression patterns that vary with diet, genetics, and their interaction, we performed expression profiling by RNA-Seq across four different tissues (liver, gonadal fat, soleus muscle, and pancreas) of TALLYHO/Jng (TH) and C57BL/6J (B6) male (M) and female (F) mice, fed either a chow or high fat (HF) diet at 5 weeks and 20 weeks of age. Expression profiling by RNA-Seq was performed on fat, liver, pancreas, and soleus muscle samples from male (M) and female (F) TALLYHO (TH) and C57BL/6J (B6) mice fed either a chow or high-fat (HF) diet. Samples were taken from mice aged 5 weeks and 20 weeks.

为识别随饮食、遗传及其交互作用而变化的基因表达模式，本研究采用RNA测序（RNA-Seq）技术，对TALLYHO/Jng（TH）与C57BL/6J（B6）品系的雄性（M）、雌性（F）小鼠的四种组织——肝脏、性腺脂肪、比目鱼肌及胰腺——开展了表达谱分析。上述小鼠在5周龄和20周龄时分别被饲喂普通饲料（chow）与高脂（HF）饲料。
本研究还针对饲喂普通饲料（chow）或高脂（HF）饲料的TALLYHO（TH）与C57BL/6J（B6）品系雌雄小鼠的脂肪、肝脏、胰腺及比目鱼肌样本，采用RNA测序技术完成了表达谱分析，样本采集自5周龄与20周龄的小鼠。