T505A variant in ETV5 promotes proliferation of precursor B cells in a mouse model of acute lymphoblastic leukemia

The goal of this project was to determine the effect of forced expression of ETS-translocation-variant-5 (ETV5) wild-type, R392P, V444I, and T505A variants on gene expression in a cultured pre-B cell line SeptMBr. Total RNA was prepared from SeptMBr interleukin-7-dependent pre-B cells transduced with control retroviral vector (MIEG3), or retroviral vectors encoding wild-type, R392P, V444I, or T505A ETV5 variants (3 biological replicates of each). This is a total of 15 biosamples. For each biosample, polyA enriched libraries were prepared and sequenced using the Illumina NovaSeq PE 100-25M reads by Genome Quebec. Each biosample consists of a BAM file containing aligned paired end reads (Mm10, GRCm38).

本项目旨在探究强制表达ETS易位变体5（ETS-translocation-variant-5，ETV5）野生型及R392P、V444I、T505A突变体对培养的前B细胞系SeptMBr中基因表达的影响。本研究从依赖白细胞介素-7（interleukin-7）的SeptMBr前B细胞中提取总RNA，这些细胞已分别被转导用对照逆转录病毒载体（MIEG3）或携带ETV5野生型、R392P、V444I、T505A变体的逆转录病毒载体，每组设置3次生物学重复，共计15个生物样本。针对每个生物样本，构建polyA富集文库，并由魁北克基因组中心（Genome Quebec）使用Illumina NovaSeq平台进行双端测序，单样本测序读长为100bp，测序量约25M reads。每个生物样本对应一份包含比对后双端reads的BAM格式文件，比对所用的参考基因组为Mm10（GRCm38）。