Table_3_Comprehensive analysis of cucumber RAV family genes and functional characterization of CsRAV1 in salt and ABA tolerance in cucumber.xls

The RAV (related to ABI3 and VP1) transcription factors are specific and exist in plants, which contain a B3 DNA binding domain and/or an APETALA2 (AP2) DNA binding domain. RAVs have been extensively studied in plants, and more and more evidences show that RAVs are involved in various aspects of plant growth and development, stress resistance and hormone signal transduction. However, the systematic analysis of RAV family in cucumber is rarely reported. In this study, eight CsRAV genes were identified in cucumber genome and we further comprehensively analyzed their protein physicochemical properties, conserved domains, gene structure and phylogenetic relationships. The synteny analysis and gene duplications of CsRAV genes were also analysed. Cis-element analysis revealed that the CsRAVs promoter contained several elements related to plant hormones and abiotic stress. Expression analysis showed that NaCl and ABA could significantly induce CsRAV genes expression. Subcellular localization revealed that all CsRAVs were localized in the nucleus. In addition, 35S:CsRAV1 transgenic Arabidopsis and cucumber seedlings enhanced NaCl and ABA tolerance, revealing CsRAV1 may be an important regulator of abiotic stress response. In conclusion, comprehensive analysis of CsRAVs would provide certain reference for understanding the evolution and function of the CsRAV genes.

RAV（与ABI3及VP1相关）转录因子是植物特有的一类转录因子，其包含B3 DNA结合结构域和/或APETALA2（AP2）DNA结合结构域。目前学界已在植物中对RAV家族开展了广泛研究，越来越多的证据表明，RAV参与植物生长发育、抗逆及激素信号转导等多个生物学过程。但目前针对黄瓜RAV家族的系统性分析研究鲜有报道。本研究从黄瓜基因组中鉴定得到8个CsRAV基因，并对其编码蛋白的理化性质、保守结构域、基因结构及系统发育关系进行了全面分析；同时还对CsRAV基因的共线性特征与基因重复事件展开了研究。顺式作用元件分析结果显示，CsRAVs基因的启动子区域含有多种与植物激素及非生物胁迫相关的调控元件。表达模式分析表明，氯化钠（NaCl）和脱落酸（ABA）可显著诱导CsRAV基因的表达。亚细胞定位实验证实，所有CsRAV蛋白均定位于细胞核中。此外，35S启动子驱动的CsRAV1转基因拟南芥及黄瓜幼苗，对氯化钠（NaCl）和脱落酸（ABA）的耐受性显著提升，这表明CsRAV1可能是植物非生物胁迫响应的重要调控因子。综上，本研究对CsRAVs家族的全面分析，可为深入解析CsRAV基因的进化历程与生物学功能提供重要参考依据。