NEDD4L mediates intestinal epithelial cell ferroptosis to restrict inflammatory bowel diseases and colorectal tumorigenesis

To explore the underlying mechanisms of NEDD4L in regulating colitis, colonic tissues from DSS-treated Nedd4lf/f VillinCre (IEC KO) mice and Nedd4lf/f (WT) littermates were subjected to RNA-sequencing analysis. Overall design: Gene expression profiling analysis of RNA-seq data from colonic tissues on the 7th day from the Nedd4lf/fVillinCre and Nedd4lf/f mice administered 2 % DSS .

为探究NEDD4L调控结肠炎的潜在分子机制，本研究对经葡聚糖硫酸钠（Dextran Sulfate Sodium, DSS）造模处理的Nedd4lf/f VillinCre（肠上皮细胞敲除，IEC KO）小鼠及其同窝野生型（Wild Type, WT）Nedd4lf/f小鼠的结肠组织开展RNA测序（RNA-sequencing）分析。实验整体设计：对给予2% DSS的Nedd4lf/fVillinCre与Nedd4lf/f小鼠，在造模第7天采集其结肠组织，对获取的RNA测序数据进行基因表达谱分析。