Comparative transcriptomics of Clostridium thermocellum wild type (?hpt) and Type I glutamine synthetase (Clo1313_2031) deletion strains using RNA-seq

RNA-seq analysis revealed that genes involved in urea uptake and metabolism were significantly upregulated in the Clo1313_2031 deletion strain, suggesting that deletion of Clo1313_2031 mimics nitrogen starvation in C. thermocellum. Additionally, genes encoding the RNF-complex were also more highly expressed and in turn may have a potential role in increasing ethanol production. Overall design: Samples for RNA-seq were taken from mid-exponential phase (OD ~ 0.33) batch cultures grown in MTC on 4.5 g/l cellobiose

RNA测序（RNA-seq）分析显示，参与尿素摄取与代谢的基因在Clo1313_2031缺失菌株中显著上调，提示敲除Clo1313_2031可模拟热纤梭菌（C. thermocellum）的氮饥饿状态。此外，编码RNF复合物（RNF-complex）的基因表达水平同样显著升高，该复合物或在提升乙醇产量方面具备潜在调控功能。实验整体设计：RNA测序所用样本取自以4.5 g/L纤维二糖为碳源、在MTC培养基中培养至指数生长中期（光密度OD≈0.33）的分批培养物。