SUMOylation fine-tunes the transcriptional activity of endothelial HEY1 in the regulation of angiogenesis [MLEC RNA-seq]

To validate the results in primary endothelial cells including HUVECs and HMVECs, the effect of HEY1 SUMOylation on angiogenic functions was examined in MLECs isolated form Hey1 iEC-KO HEY1-WT/3KR iEC-KI mice. Overall design: Samples were MLECs isolated form Hey1 iEC-KO HEY1-WT/3KR iEC-KI mice. Six samples were divided into two groups: HEY1-WT iEC-KI group and HEY1-3KR iEC-KI group. There are 3 replicates in each group.

为在包括人脐静脉内皮细胞（HUVECs）与人微血管内皮细胞（HMVECs）在内的原代内皮细胞（primary endothelial cells）中验证实验结果，本研究在分离自Hey1诱导型内皮细胞特异性敲除（iEC-KO）且携带HEY1野生型（HEY1-WT）/3KR敲入（iEC-KI）小鼠的小鼠肺微血管内皮细胞（MLECs）中，检测了HEY1的SUMO化修饰（SUMOylation）对血管生成功能的影响。整体实验设计如下：实验样本为分离自Hey1 iEC-KO HEY1-WT/3KR iEC-KI小鼠的MLECs；共6份样本分为两组，即HEY1-WT iEC-KI组与HEY1-3KR iEC-KI组，每组设置3个生物学重复。