TLS Dataset

Tertiary lymphoid structures (TLS) dataset.

Histological cases were retrieved from the archive of the Norfolk and Norwich University Hospital histopathology department. Tumours were classified according to the 2015 WHO classification. For each patient, TLS assessment was based on a representative tissue block, with adequate tumour material and interface between normal and tumor tissue well represented. Annotation was performed on tumour tissue slides from 18 patients with primary lung cancer. There were 8 tumours, of which there were 14 adenocarcinoma (5 acinar predominant, 1, papillary, 2 solid predominant and 6 lepidic predominant), 3 squamous cell carcinomas and 2  sarcomatoid carcinomas. The age range of the patients was 54-82 years old, with an average of 69 ± 2.4 years.  The tumour size was between 8 and 62 mm, with an average of 28 ± 6.4. TLS was defined as all dense lymphocytic aggregates and 144 TLS were annotated by 2 histopathologists (FK, MDC) with 100% concordance. To assess the generalizability of the model, this dataset was created for internal training-validation and further validation in independent populations.

Please cite as:

Barmpoutis P, Di Capite M, Kayhanian H, Waddingham W, Alexander DC, Jansen M, et al. (2021) Tertiary lymphoid structures (TLS) identification and density assessment on H&E-stained digital slides of lung cancer. PLoS ONE 16(9): e0256907. https://doi.org/10.1371/journal.pone.0256907

三级淋巴结构（Tertiary lymphoid structures, TLS）数据集。

本研究的组织学病例调取自诺福克与诺里奇大学医院病理科档案。肿瘤依照2015年世界卫生组织（WHO）分类标准进行分型。每例患者的TLS评估均基于具有代表性的组织蜡块，该蜡块需包含充足的肿瘤组织，且正常组织与肿瘤组织的交界区域得到充分显示。

研究对18例原发性肺癌患者的肿瘤组织切片完成注释，所涉及的肿瘤共8例，其中包括14例肺腺癌（5例以腺泡结构为主、1例以乳头状结构为主、2例以实体结构为主、6例以鳞屑样结构为主）、3例鳞状细胞癌以及2例肉瘤样癌。患者的年龄范围为54~82岁，平均年龄为69±2.4岁。肿瘤直径介于8~62 mm之间，平均直径为28±6.4 mm。

TLS被定义为所有致密淋巴细胞聚集灶，由2名病理医师（FK、MDC）完成144个TLS的注释，二者的注释一致性达100%。为评估模型的泛化能力，本数据集被用于模型的内部训练与验证，以及独立人群队列的进一步验证。

请引用如下：

Barmpoutis P, Di Capite M, Kayhanian H, Waddingham W, Alexander DC, Jansen M 等. (2021) 肺癌H&E染色数字化切片中三级淋巴结构的识别与密度评估. PLoS ONE 16(9): e0256907. https://doi.org/10.1371/journal.pone.0256907