3. Impact of Coaggregation of Streptococcus gordonii-Streptococcus oralis in Expression of genes using Dual RNA-Seq approach

When two oral bacteria are allowed to grow together, they physically interact and adhere among themselves to display specific recognition patterns. This recognition between genetically distinct cells in suspension and resultant clumping is called coaggregation.These intergenic pairings called dual biofilms are important in the sequential formation of oral biofilms. So, our study is limited to dual biofilms. coaggregation lead to specific gene regulation responses. Mutualistic interactions allow growth of mixed species communities.This study aimed to explore the impact of coaggregation between S. gordonii and S. oralis in human saliva on the gene expression in each partner. Understanding responses to coaggregation will provide important insights into how these species adapt as they interact with one another during the formation of oral biofilms.

当两种口腔细菌共同培养时，二者会发生物理相互作用并彼此黏附，呈现出特定的识别模式。悬浮状态下遗传背景各异的细胞之间的此类识别作用及由此产生的聚集现象，被称为共凝集（coaggregation）。这种被称为双生物膜（dual biofilms）的双菌种配对，在口腔生物膜的序贯形成过程中发挥着关键作用。因此本研究仅聚焦于双生物膜体系。共凝集可触发特异性的基因调控应答，而互利共生相互作用能够促进混合物种群落的生长。本研究旨在探究人类唾液中戈登链球菌（Streptococcus gordonii，缩写S. gordonii）与口腔链球菌（Streptococcus oralis，缩写S. oralis）之间的共凝集，对双方各自基因表达的影响。阐明共凝集相关的应答机制，将有助于深入理解这两种菌种在口腔生物膜形成过程中彼此相互作用时的适应策略。