Microscopy image data of a titration series with DNA surface strands and condensate-forming DNA nanomotifs (repeat)

The dataset contains microscopy image data of DNA-nanomotifs (color channel 2) that can form liquid-like condensates, especially in association with target DNA strands that can act as condensation surfaces (color channel 1). The data were recorded on a VisiTech iSIM microscopy with a 40X water immersion objective (Apo LWD 40x WI λS DIC N2, NA 1.15). Images were recorded using dual ORCA-Flash4.0 V3 cameras with simultaneous two-channel acquisition to avoid displacement of objects due to consecutive acquisition of color channels. The file names contain the flow channel number, the presence of condensation surfaces (SX4), and the amount of added nanomotif solution in microliters (NX in µl).

The image files are in the .nd2 Nikon proprietary format, but can be read with the OME bioformats libraries.

Scripts for the analysis of these data are available via a GitHub repository: https://github.com/lhilbert/SurfacesAndCondensates

This repository contains data recorded on 10 March, 2023

The file size of data were too large, so additional repositories were opened. The complete date need to be also downloaded from these additional repositories:

EXTRA DATA 1/2:  10.5281/zenodo.13788977

https://doi.org/10.5281/zenodo.13788977

EXTRA DATA 2/2: 10.5281/zenodo.13789555

https://doi.org/10.5281/zenodo.13789555

There is another data set that contains a repeat of the same conditions, recorded on 13 March, 2023, under the following link

https://doi.org/10.5281/zenodo.13788484

本数据集包含可形成类液凝聚体（liquid-like condensates）的DNA纳米基序（DNA-nanomotifs）的显微镜图像数据，其中DNA纳米基序由色通道2标记；可作为凝聚表面（condensation surfaces）的靶DNA链（target DNA strands）则由色通道1标记。数据采集使用VisiTech iSIM显微镜，搭配40倍水浸物镜（Apo LWD 40x WI λS DIC N2，数值孔径NA 1.15）。图像采集采用双ORCA-Flash4.0 V3相机进行双通道同步采集，以避免因依次采集不同色通道导致的目标位移。文件名包含流道编号、凝聚表面（SX4）的有无，以及添加的纳米基序溶液体积（单位为微升，记为NX，单位µl）。

图像文件采用尼康专属的.nd2格式，但可通过OME生物格式库（OME bioformats libraries）读取。

本数据集的分析脚本可通过GitHub仓库获取：https://github.com/lhilbert/SurfacesAndCondensates。该仓库包含2023年3月10日采集的实验数据。

由于原始数据文件体积过大，因此设立了额外仓库。完整数据集需从以下额外仓库下载：
补充数据集1/2：10.5281/zenodo.13788977，DOI：10.5281/zenodo.13788977
补充数据集2/2：10.5281/zenodo.13789555，DOI：10.5281/zenodo.13789555

另有一组重复相同实验条件的数据集，采集于2023年3月13日，可通过以下链接获取：https://doi.org/10.5281/zenodo.13788484