Genome wide mapping of histone variants in C2C12 cells. Mus musculus

The project aims to reveal biological function of histone variants. The samples are the C2C12 cells that exogenously produce the GFP-tagged histone variants and also contains wild-type cells. We performed ChIP-Seq for the tags and epigenetic markers. We also performed mRNA-Seq to profile the gene expression in the cells.

本项目旨在揭示组蛋白变体（histone variants）的生物学功能。实验样本包含外源性表达绿色荧光蛋白（GFP）标记的组蛋白变体的C2C12细胞，以及野生型细胞。本研究针对上述样本开展了染色质免疫沉淀测序（ChIP-Seq），以分析标签蛋白与表观遗传标记的结合特征；同时通过mRNA测序（mRNA-Seq）对细胞的基因表达谱进行解析。