Interactions between gamma-delta T lymphocytes and M-MDSC cells in the pathogenesis of chronic lymphocytic leukemia

RNA-seq of zoledronate-expanded Vdelta2 gamma-delta T cells from healthy donors, cultured alone or co-incubated with myeloid-derived suppressor cells (M-MDSC) or vitamin C. The study investigates how M-MDSC influence gamma-delta T-cell function and differentiation, and the potential modulatory effects of vitamin C and vitamin D. These data provide insight into immune regulation in CLL and mechanisms relevant for immunotherapy development. This study investigated the effect of myeloid-derived suppressor cells (M-MDSC) and vitamin C on the transcriptomic profile of zoledronate-expanded Vδ2 γδ T cells from healthy human donors. Peripheral blood γδ T cells were expanded in vitro with zoledronate and IL-2, then either cultured alone (control), co-incubated with autologous M-MDSC, or treated with vitamin C. Total RNA was extracted and subjected to paired-end RNA sequencing (150 bp) using the DNBSEQ-T7 platform (MGI Tech). Differential expression analysis was performed to compare gene expression between experimental conditions.

本数据集为健康供者来源的经唑来膦酸（zoledronate）扩增的Vδ2 γδ T细胞的RNA测序数据，相关细胞分别接受单独培养、与髓系来源抑制细胞（myeloid-derived suppressor cells, M-MDSC）共培养，或经维生素C处理。本研究旨在探究M-MDSC对γδ T细胞功能与分化的调控作用，以及维生素C与维生素D的潜在调节效应，相关数据可为慢性淋巴细胞白血病（chronic lymphocytic leukemia, CLL）中的免疫调控机制及免疫治疗开发相关通路提供研究视角。
本研究针对健康人类供者来源的经唑来膦酸扩增的Vδ2 γδ T细胞，探究了M-MDSC与维生素C对其转录组谱的影响：研究人员先通过唑来膦酸与白细胞介素2（interleukin-2, IL-2）体外扩增外周血γδ T细胞，随后将扩增后的细胞分为三组，分别为单独培养的对照组、与自体M-MDSC共培养组，以及维生素C处理组。随后提取总RNA，使用DNBSEQ-T7平台（MGI Tech）开展150bp双端RNA测序，并通过差异表达分析比较不同实验分组间的基因表达差异。