Transcriptional decay profiling of Neurospora crassa

Codon optimality mediated decay(COMD) is a conserved phenomenon from yeast to human. Our data suggests that COMD alson exist in neurospora crass. We resort thiolutin(inhibitor for transcription) to treat cells in different time point and transcriptionally profiled them to get the landscape of transpcriptional decay profiling. 24h cultured cell under constant light were treat with thiolutin(12μg/ml), then cell were harvested from time point 0min, 20min, 40min, 60min.

密码子最优性介导的衰变（Codon optimality mediated decay, COMD）是一类从酵母到人类均保守存在的生物学现象。本研究数据显示，COMD在粗糙脉孢菌（Neurospora crassa）中同样存在。我们使用硫代菌素（一种转录抑制剂）对不同时间点的细胞进行处理，并通过转录组分析获取转录衰变全景图谱。将经持续光照培养24小时的细胞以12μg/ml浓度的硫代菌素处理后，分别于0min、20min、40min、60min四个时间点收集细胞。