ALI1 interacts with N-acyl homoserine lactone and is involved in the perception of the quorum sensing molecule

In this report, we investigated oxo-C14-HSL-interacting proteins in plants. We propose the AHL-Interacting Protein 1 (ALI1) (previously known as AtGalk2) as interacting protein for oxo-C14-HSL in Arabidopsis. We present here several levels of confirmation and suggest that ALI1 is required for the perception of oxo-C14-HSL in Arabidopsis. This is the first direct evidence of a plant protein interacting with a bacterial quorum sensing molecule. Examination of Col-0 and ali1 mutant seedlings pretreated for three days with 6 µM oxo-C14-HSL or acetone control and subsequently elicited with 100 nM flg22. All treatments were performed in triplicates.

本研究针对植物中与氧代-C14-高丝氨酸内酯(oxo-C14-HSL)互作的蛋白开展了系统性探究。我们提出，此前被命名为AtGalk2的AHL互作蛋白1(ALI1)可作为拟南芥(Arabidopsis)中与oxo-C14-HSL互作的靶蛋白。本文呈现了多维度的验证结果，并指出ALI1是拟南芥感知oxo-C14-HSL过程中的必需蛋白。这是首个关于植物蛋白与细菌群体感应分子互作的直接实验证据。本实验以6 μM oxo-C14-HSL或丙酮对照预处理Col-0野生型与ali1突变体幼苗3天，随后采用100 nM鞭毛蛋白22(flg22)进行诱导处理。所有实验处理均设置三次生物学重复。