Differential expression of genes in pancreatic islet of C57Bl/6J on high fat diet. Mus musculus

Male C57Bl/6J mice were fed 45%kcal fat diet (HF) or regular rodent chow (NC) from 4 weeks to 16 weeks of age. Gene expression was compared between RNA obtained from pancreatic islets of HF fed mice and NC mice. Overall design: RNA samples from 4 NC group and 4 HF groups were analyzed using GeneChip Mouse Expression Arrays MOE 430v2 (Affymetrix).

雄性C57Bl/6J小鼠自4周龄至16周龄期间，分别饲喂45%热量占比的脂肪饮食（高脂饮食，high-fat diet，HF）与普通啮齿类饲料（正常饲料，normal chow，NC）。提取两组小鼠胰岛的RNA，比较其基因表达水平差异。
实验设计：采用Affymetrix公司的GeneChip小鼠表达谱芯片MOE 430v2，对4份正常饲料组RNA样本与4份高脂饲料组RNA样本进行检测分析。