Determination of the metabolic profile of Solidago canadensis using UFLC-PDA-ESI-TOF

Abstract Species in the Solidago genus are used worldwide due to the therapeutic activities of their flavonoids and terpenoids. Its anti-inflammatory, antirheumatic, analgesic, diuretic, aquaretic, wound-healing properties as well as its ability to relieve burn and insect bites allow use in a variety of situations. This study was carried out to determine the metabolic profile of Solidago canadensis inflorescences (SCAI) to identify the species commercialized in Rio de Janeiro, RJ, Brazil, and evaluate the medicinal potential compared to S. chilensis and SCAI, which is used in North America and Europe. The UFLC-PDA-ESI-TOF revealed the metabolic profile of SCAI, finding the pseudo-molecular ions and characteristic fragments, mainly flavonols (10) such as quercetin, kaempferol, isorhamnetin, and its glycosides, as well as chlorogenic acid (CA). Eleven substances were identified, confirming the compatibility of the metabolic profile presented in varieties canadensis and scabra of european S. canadensis. The both contain the same flavonoid aglycones, and their glycosides are important chemical and biological markers for extracts and products based on species of the genus Solidago. UFLC-PDA-ESI-TOF characterized the extracts, which can help with quality control of raw plant materials and commercialized phytotherapeutics as well as for development of new products and phytomedicines.

摘要 一枝黄花属（Solidago）植物因其所含黄酮类与萜类成分的治疗活性而在全球范围内得到广泛应用。该属植物具备抗炎、抗风湿、镇痛、利尿、利水、促进伤口愈合的功效，同时可缓解烧伤与昆虫叮咬引发的不适，因此可应用于多种临床场景。本研究旨在解析加拿大一枝黄花（Solidago canadensis）花序（SCAI）的代谢谱，以鉴定巴西里约热内卢州（RJ）商业化流通的一枝黄花属物种，并与智利一枝黄花（S. chilensis）以及欧美地区常用的SCAI进行对比，评估其药用潜力。本研究采用超高效液相色谱-光电二极管阵列-电喷雾电离-飞行时间质谱（UFLC-PDA-ESI-TOF）对SCAI的代谢谱进行分析，检测到伪分子离子与特征碎片峰，主要包括10种黄酮醇类成分（如槲皮素、山奈酚、异鼠李素及其苷类）以及绿原酸（CA）。本研究共鉴定出11种物质，证实欧洲加拿大一枝黄花的加拿大变种与糙叶变种的代谢谱与本次检测结果具有一致性。二者拥有相同的黄酮苷元，其苷类可作为一枝黄花属植物提取物及相关产品的重要化学与生物学标志物。UFLC-PDA-ESI-TOF可实现提取物的成分表征，该技术可用于植物原材料与商业化植物治疗药物的质量控制，同时助力新型产品与植物药的研发。