Chromatin response to hypoxia in MCF7 cells. Chromatin response to hypoxia in MCF7 cells

Oxygen is essential for cell growth, however in tumours quick growth can cause hypoxic conditions. To determine the changes in the chromatin landscape in response to hypoxia in breast cancer we have performed ATAC-seq dugin normoxia and hypoxia. Overall design: Biological replicates with one or two technical replicates were grown for 48 hours in hypoxic or normoxic conditions before ATAC-seq.

氧气是细胞生长所必需的物质，而肿瘤的快速增殖可引发缺氧（hypoxia）微环境。为探明乳腺癌细胞染色质景观（chromatin landscape）对缺氧的响应变化，我们分别在常氧（normoxia）与缺氧（hypoxia）条件下开展了转座酶可及性测序（ATAC-seq）实验。实验整体设计：我们设置了多组生物学重复，每组生物学重复搭配1至2个技术重复，将细胞在常氧或缺氧环境中培养48小时后，再进行ATAC-seq检测。