ZIC2 Is Required For Nodal Expression And The Establishment Of Left-Sided Identity. ZIC2 Is Required For Nodal Expression And The Establishment Of Left-Sided Identity

The purpose of this study was to identify putative downstream targets of the transcription factor ZIC2 in the mouse embryo. The results indicate loss of NODAL pathway expression, consistent with the observed phenotype of right isomerism in heart, lungs and viscera. Overall design: Total RNA obtained from wildtype whole embryos at day E8.0-E8.5 was compared to that from mutant Zic2 iso/iso embryos

本研究旨在鉴定小鼠胚胎中转录因子ZIC2的推定下游靶标。研究结果显示NODAL信号通路（NODAL pathway）的表达缺失，该结果与在心脏、肺及内脏中观察到的右侧异构表型相符。实验整体设计：将采集自野生型（wildtype）胚胎发育第8.0至8.5天（E8.0-E8.5）完整胚胎的总RNA（Total RNA），与Zic2 iso/iso突变胚胎的总RNA进行对照分析。