From transcriptomics to phenomics to reveal adaptation response of Lactobacillus plantarum C2 in plant substrates. Lactiplantibacillus plantarum

The aim of the study was to determine biological relevance of differentially expressed genes in Lactobacillus plantarum C2 during fermentation of plant substrates. Whole-transcriptome analysis based on customized microarray profiles has been used to determine altered transcription patterns in L. plantarum C2. Overall design: L. plantarum C2 was grown (24 h at 30°C) and stored ( 21 days at 4°C) in carrot or pineapple juices to mimic the chemical composition of the respective raw matrices. De Man, Rogosa and Sharpe broth was used as the control medium for optimal growth. Samples were analyzed at the late exponential phase of growth and after storage

本研究旨在探究植物乳杆菌C2（Lactobacillus plantarum C2）在植物基质发酵过程中差异表达基因（differentially expressed genes）的生物学相关性。本研究采用基于定制化微阵列（microarray）谱的全转录组分析（whole-transcriptome analysis）技术，解析植物乳杆菌C2的转录模式变化。
实验设计：将植物乳杆菌C2于30℃下培养24小时，随后分别置于胡萝卜汁与菠萝汁中于4℃储存21天，以模拟对应原料基质的化学成分；德-罗-沙肉汤（De Man, Rogosa and Sharpe broth）作为最优生长的对照培养基。分别于生长后期指数期及储存结束后采集样本进行分析。