microRNAs profiling in hippocampal cells of mouse with Sound-induced Seizure

After an electrically induced seizure, mice (NMRI) received electrical stimulation (current intensity 44 mA, 0.2 ms monopolar pulses at 6 Hz frequency for 3 s). RNA was extracted from the hippocampi at 0, 3, 6, 24 and 72 h after the seizure. microRNA expressions were measured via microarray technology using Exiqon's miCURY™ LNA Arrays. Time-course design, treated cells at 0, 3, 6, 24, and 72 h, with 7 replicate each

电诱导癫痫发作完成后，对NMRI品系小鼠施加电刺激：刺激参数为电流强度44 mA、0.2 ms单极脉冲、频率6 Hz，刺激时长3秒。于癫痫发作后0、3、6、24及72小时，从小鼠海马组织中提取RNA。通过基因芯片（microarray）技术，使用艾克松（Exiqon）公司的miCURY™锁核酸（LNA）芯片检测微小RNA（microRNA）的表达水平。本实验采用时间序列设计，在上述5个时间点采集处理后的样本，每个时间点设置7个生物学重复。