Oncogenic KRAS-dependent stromal interleukin-33 directs the pancreatic microenvironment to promote tumor growth [scRNA-seq]

Here, we sought to understand the function of stromal interleukin-33 (IL-33) in PanIN and PDA. We collected pancreatic tissues from various mouse models of PanIN and PDA for single cell RNA sequencing to assess IL-33 expression across the microenviornment. We also implanted syngeneic orthotopic pancreatic tumors into mice harboring a conditional stromal IL-33 knockout and sequenced the resulting tumors. Pancreata from various mouse models of pancreatic tumorigenesis (spontaneous or orthotopic injection) collected and subjected to single cell RNA sequencing.

本研究旨在阐明基质白细胞介素33（IL-33）在胰腺上皮内瘤变（PanIN）及胰腺导管腺癌（PDA）中的功能。我们收集了多种PanIN与PDA小鼠模型的胰腺组织，开展单细胞RNA测序，以评估IL-33在肿瘤微环境中的表达情况。此外，我们向携带条件性基质IL-33敲除的小鼠体内植入同基因原位胰腺肿瘤，并对生成的肿瘤进行测序。同时，我们收集了多种胰腺肿瘤发生小鼠模型（包括自发性模型与原位注射模型）的胰腺组织，并进行单细胞RNA测序。