Diversity of ridged frogs (Anura: Ptychadenidae: Ptychadena spp.) in wetlands of the upper Nile in Rwanda: morphological, bioacoustic, and molecular evidence

We investigated the distribution of species of the genus Ptychadena at nine sites at the upper Nile and its catchment in Rwanda. For species delimitation, we chose an integrative approach, combining morphological and bioacoustic data and DNA barcoding (mitochondrial 16S rRNA gene). We identified three species using independent evidence from the three different data sets: Ptychadena anchietae, Ptychadena porosissima, and a species of the Ptychadena mascareniensis group. The latter is undistinguishable genetically, bioacoustically, and morphologically from populations from Uganda, Kenya, and Egypt. We resurrect the name Ptychadena nilotica for these populations. The species differs strongly genetically from topotypic P. mascareniensis, and from clades referred to as P. cf. mascareniensis from Western and Central Africa. Morphologically, the three Rwandan species can be differentiated by their quantitative morphometrics (discriminant analysis, success rate: 98.3%) and by a number of qualit...

我们针对卢旺达境内尼罗河上游及其集水区的9个采样点，开展了褶蛙属（Ptychadena）物种分布的调查研究。在物种界定方面，我们采用整合性研究方法，结合形态学、生物声学数据与DNA条形码技术（线粒体16S核糖体RNA基因）。通过来自三类不同数据集的独立证据，我们共鉴定出3个物种：Ptychadena anchietae、Ptychadena porosissima，以及属于Ptychadena mascareniensis物种群的一个物种。该类群与乌干达、肯尼亚及埃及的种群在遗传学、生物声学及形态学特征上均无法区分。我们将Ptychadena nilotica这一学名重新启用以指代这些种群。该物种与模式产地的P. mascareniensis以及西非、中非地区被称为P. cf. mascareniensis的进化枝在遗传学上存在显著差异。在形态学上，卢旺达的这3个物种可通过其量化形态测量数据（判别分析，准确率达98.3%）以及一系列定性……