Combinatorial effect of serotonin and indole on enterohemorrhagic Escherichia coli (EHEC) transcriptome

Previous studies have shown serotonin and indole downregulate expression of virulence genes in EHEC, here we demonstrate the combinatorial effect of serotonin and indole on EHEC transcriptome Overall design: EHEC strain 86 24 was used for this study. WT EHEC was grown with 10 µM serotonin, 500 µM indole or both of them together in low-glucose DMEM until late log phase under aerobic conditions. RNA was extracted from these samples. The experiment was conducted with three biological replicates.

既往研究表明，5-羟色胺（serotonin）与吲哚（indole）可下调肠出血性大肠杆菌（Enterohemorrhagic Escherichia coli，EHEC）的毒力基因表达，本研究则阐明二者对EHEC转录组的联合调控效应。实验设计：本研究采用EHEC菌株86 24。将野生型（wild type，WT）EHEC分别接种于添加10 μM 5-羟色胺、500 μM 吲哚，以及二者联合的低糖杜氏改良伊格尔培养基（low-glucose DMEM）中，于有氧条件下培养至对数生长后期。收集各样本并提取总RNA。本实验设置3次生物学重复。