FOXC1 in triple negative breast cancer [ChIP-seq]

To investigate FOXC1 chromatin binding, and the effect of FOXC1 CRISPR knockout in triple negative breast cancer cell lines MDA-MB-231, MDA-MB-468, Hs578t, and Bt-549. Overall design: We performed RNA-seq on WT and FOXC1 KO lines, and ChIP-seq for FOXC1 on WT cell lines.

本研究旨在探究FOXC1的染色质结合特性，以及FOXC1的CRISPR敲除对三阴性乳腺癌细胞系MDA-MB-231、MDA-MB-468、Hs578t及Bt-549的影响。
整体实验设计：本研究对野生型（WT）与FOXC1基因敲除（KO）细胞系开展了RNA测序（RNA-seq），并在野生型细胞系中针对FOXC1完成了染色质免疫沉淀测序（ChIP-seq）。