Whole-genome sequencing of Tisochrysis lutea strains for the DynAlgue genome-wide association study, 2020

In this study, Genome-Wide Association Studies (GWAS) were challenging to decipher genomic loci without a priori assumptions. Microalgal Tisochrysis lutea was selected as a case study due. Particular focus was placed on pigment and lipid metabolism. To implement the GWAS approach, a collection of algal lineages was established from poly-clonal strains available. This collection was then phenotyped and concurrently, the genome of each lineage was sequenced.

本研究中，若缺乏先验假设，全基因组关联分析（Genome-Wide Association Studies, GWAS）在解析基因组位点时往往颇具挑战。
研究选取微藻卢特异囊藻（Tisochrysis lutea）作为案例研究对象，具体原因在于。
本研究特别关注色素与脂类代谢通路。
为开展该全基因组关联分析，研究人员从现有多克隆菌株中构建了一套藻类谱系集合。
随后对该谱系集合进行表型鉴定，同时对每个谱系的基因组进行测序。