Peripheral blood mononuclear cells as a source of cold exposure biomarkers

Male ferrets, aged 3 months, were divided into two group: one group remained at 22 degrees Celsius, while the other group was acclimatized to 4 degrees Celsius for one week. After sacrification, inguinal and periaortic white adipose tissues were dissected, and peripheral blood mononuclear cells (PBMC) were isolated. The three tissues are used for RNA isolation and subsequent global gene expression profiling using custom Agilent ferret-specific 2x400K microarrays. Data analysis indicated that the cold exposure induce a clear gene expression response of some genes in the inguinal and periaortic white adipose tissue as in PBMC. These genes could be defined as a biomakers of the effect of cold exposure in these cells. Ferrets, male, aged 3 months, were divided into two groups: one group remained at 22 degrees Celsius, while the other group was acclimatized to 4 degrees Celsius for one week. After sacrification, peripheral blood mononuclear cells (PBMC) were isolated and immediately snap frozen in liquid nitrogen. Total RNA was isolated, quantified and qualified, and subsequently used for global gene expression profiling using custom Agilent ferret-specific 2x400K microarrays.

3月龄雄性雪貂被分为两组：一组维持在22℃环境中，另一组在4℃环境中适应1周。处死后，解剖分离腹股沟白色脂肪组织与主动脉旁白色脂肪组织，并分离外周血单个核细胞（peripheral blood mononuclear cells, PBMC）。取上述三种组织进行RNA提取，随后使用定制化安捷伦雪貂特异性2×400K基因芯片开展全基因组基因表达谱分析。数据分析显示，冷暴露可在腹股沟及主动脉旁白色脂肪组织与外周血单个核细胞中诱导部分基因产生显著的表达应答，此类基因可作为上述组织中冷暴露效应的生物标志物。
3月龄雄性雪貂被分为两组：一组维持在22℃环境中，另一组在4℃环境中适应1周。处死后，分离外周血单个核细胞并立即置于液氮中快速冷冻。对总RNA进行提取、定量及质控，随后使用定制化安捷伦雪貂特异性2×400K基因芯片开展全基因组基因表达谱分析。