Knockdown of Akt1/2 by CRISPR/Cas9 suppresses the metastasis of human prostate cancer cells CWR22rv1 in vitro and in vivo

Our findings will initially map out the downstream signal pathways of Akt and AR mutual feedback to regulate CaP metastasis through the study of these scientific assumptions, which will provide targets for the development of new precise treatments for prostate cancer. Overall design: we knockout Akt1 and Akt2 genes in CaP CWR22rv1 cells to study effects of Akt1/2 knockout on metastasis in CaP CWR22rv1 cells in vitro and in vivo.

本研究通过对上述科学假设的探究，首次系统阐明Akt与雄激素受体（Androgen Receptor, AR）相互反馈调控前列腺癌（Cancer of the Prostate, CaP）转移的下游信号通路，可为前列腺癌新型精准治疗的研发提供潜在靶点。
整体实验设计：我们在CaP CWR22rv1细胞中敲除Akt1与Akt2基因，分别在体外与体内环境中探究Akt1/2基因敲除对CaP CWR22rv1细胞转移能力的影响。