Identification of miR-210 target genes in T20 patient-derived sphere culture. Homo sapiens

Hypoxia is known to regulate tumor-initiating cells and to have an effect on miRNA expression. We were interested in studying the role of hypoxia-induced miR-210 in colorectal cancer patient-derived sphere cultures. Downregulated genes after overexpression of miR-210 were retained as potential miR-210 target genes for further validation sudies. Overall design: Colorectal cancer patient-derived (patient name: T20) sphere cultures were stably transduced with miR-210 lentiviral particles (Biosettia) or control vectors, respectively. Three technical replicates were used for both conditions.

已知缺氧（Hypoxia）可调控肿瘤起始细胞，并对微小RNA（microRNA，miRNA）的表达产生影响。本研究旨在探讨缺氧诱导的miR-210在结直肠癌患者来源的肿瘤球培养物中的功能作用。将miR-210过表达后发生下调的基因保留为潜在的miR-210靶基因，用于后续验证研究。实验整体设计：分别将结直肠癌患者来源（患者编号：T20）的肿瘤球培养物，采用miR-210慢病毒颗粒（Biosettia公司）或对照载体进行稳定转染；两种处理条件均设置三次技术重复。