Cerebral Venous Blood Flow Regulates Brain Fluid Clearance via Dural Lymphatics. Cerebral Venous Blood Flow Regulates Brain Fluid Clearance via Dural Lymphatics

Previous studies have shown that the vascular system regulates brain clearance via arterial blood flow and the lymphatic drainage of CSF. Here, we examined the hypothesis that cerebral venous blood flow may participate in brain clearance as an intermediate compartment between the arterial and lymphatic poles of the vascular system. Transcriptomic analyses provided insights into the molecular mechanisms driving dural lymphatic remodeling within the dura mater. Overall design: Mice after sham surgery (controls) as well as mice 14 days after jugular venous ligature were used. Skullcaps were dissected and dural meninges were carefully peeled off the skullcaps. Viable singlets were isolated by flow cytometry and scRNA-seq analysis was performed.

既往研究证实，血管系统可通过动脉血流与脑脊液(Cerebrospinal Fluid, CSF)的淋巴引流途径调控大脑清除功能。本研究旨在验证下述假说：脑静脉血流可作为血管系统动脉端与淋巴端之间的中间隔室，参与大脑清除过程。转录组分析揭示了硬脑膜(Dura mater)内硬脑膜淋巴重塑的分子机制。实验设计：本研究使用两组小鼠，分别为假手术处理的对照组小鼠，以及颈静脉结扎术后14天的实验组小鼠。研究人员分离颅骨外板，并小心地从颅骨外板上剥离硬脑膜；通过流式细胞术分离得到活单细胞悬液，并开展单细胞RNA测序(Single-cell RNA sequencing, scRNA-seq)分析。