Differentially expressed miRNA in burned murine skin tissue compared to normal skin tissue. Mus musculus

Interactions between stromal cell-derived factor-1α (SDF-1α) and its cognate receptor CXCR4 are crucial for the recruitment of mesenchymal stem cells (MSCs) from bone marrow (BM) reservoirs to damaged tissues for repair during alarm situations. MicroRNAs are differentially expressed in stem cell niches, suggesting a specialized role in stem cell regulation. Here, we gain insight into the molecular mechanisms involved in regulating SDF-1α. Individualized outcome prediction classifiers were successfully constructed through expression profiling of microRNAs (in all organisms as annotated in Sanger miRBase Release 11.0 (http://microrna.sanger.ac.uk))in one burned murine skin tissue compared to normal skin tissue,which had 57 upregulated microRNAs and 28 down-regulated microRNAs. Overall design: In the study presented here, a consecutively operated, well-defined cohort of 2 cases, was used to acquire expression profiles of microRNAs (in all organisms as annotated in Sanger miRBase Release 11.0 (http://microrna.sanger.ac.uk)), leading to the successful construction of supervised. The most recent version of the array (v.11.0 - hsa, mmu & rno array) contains more than 1700 capture probes, covering all microRNAs annotated in miRBase 11.0, as well as all viral microRNAs, related to these species.

基质细胞衍生因子-1α（stromal cell-derived factor-1α，SDF-1α）与其同源受体CXCR4的相互作用，在机体应激警报状态下，对于将骨髓（bone marrow，BM）储备库中的间充质干细胞（mesenchymal stem cells，MSCs）招募至受损组织以完成修复至关重要。
微小RNA（microRNAs，miRNAs）在干细胞微环境中呈差异表达，提示其在干细胞调控中发挥特异性功能。本研究旨在深入解析调控SDF-1α的分子机制。
本研究通过对比烧伤小鼠皮肤组织与正常皮肤组织的微小RNA表达谱（所有收录于桑格研究所miRBase第11.0版（http://microrna.sanger.ac.uk）的微小RNA），成功构建了个体化预后预测分类器；其中烧伤组皮肤组织中共鉴定出57个上调微小RNA与28个下调微小RNA。
实验总体设计：本研究纳入连续收治、队列界定清晰的2例样本，通过检测收录于桑格研究所miRBase第11.0版（http://microrna.sanger.ac.uk）的所有微小RNA的表达谱，成功构建了监督分类器。
该芯片的最新版本（v.11.0 - 涵盖人、小鼠及大鼠的芯片）包含超过1700条捕获探针，可覆盖miRBase 11.0中注释的全部微小RNA以及上述物种相关的所有病毒微小RNA。