Dataset for: Towards canine immunotherapy models: Monoclonal antibodies with redox regulated epitopes targeting TIM3 attenuate Galectin-9 binding

This dataset contains the experimental data and supporting materials associated with the study "Towards canine immunotherapy models: Monoclonal antibodies with redox regulated epitopes targeting TIM3 attenuate Galectin-9 binding" . The dataset includes flow cytometry measurements of TIM3 expression in HCT116 cell lines, protocols for transient and stable transfection, plasmid assembly files, and nucleotide sequences of antibody production constructs (TIM3-IgG26, TIM3-IgG29, TIM3-scFcFv11, and TIM3-scFcFv26). These materials support the validation of anti-canine TIM3 antibodies and the characterization of TIM3 expression used in the study.本数据集包含与研究《迈向犬类免疫治疗模型：靶向T细胞免疫球蛋白黏蛋白3（TIM3）的氧化还原调控表位单克隆抗体可抑制半乳凝素-9（Galectin-9）结合》相关的实验数据及配套研究材料。数据集涵盖HCT116细胞系中TIM3表达的流式细胞术检测数据、瞬时转染与稳定转染实验方案、质粒构建文件，以及抗体生产构建体（TIM3-IgG26、TIM3-IgG29、TIM3-scFcFv11及TIM3-scFcFv26）的核苷酸序列。上述材料可用于验证抗犬类TIM3单克隆抗体，并为本研究中TIM3表达的表征工作提供支撑。