Putative mitosomal proteins classified by predicted function: miscellaneous.

Mascot score, Mascot total ion score for the identified protein. Coverage, number of unique peptides per identified protein. MiD, mitosomal distribution. Proteins are marked “Y” if their distributions in fractions #3 and #4 of the Optiprep gradient (measured by the iTRAQ ratio) were within the range between Cpn10 and IscU and the window that extended in both directions by half of the distance between these markers. Proteins with ratios outside of this range are indicated with “N”. TargetP and PsortII were used to predict the subcellular location of Giardia proteins. S, secretory; N, non-secretory; M, mitochondrial; O, other. Exp. ver., experimental verification of protein localization using the pONDRA expression vector. The recombinant tagged proteins were localized by fluorescence microscopy. M, mitosome; ER, endoplasmic reticulum; O, other; ? inconclusive. MEMSAT3 and TMHMM were used to predict transmembrane domains. SGP, predicted soluble proteins are marked with number sign (#). Asterisk (*) is used where no data were available. (**) transformed Giardia did not express the recombinant tagged protein.

Mascot评分（Mascot score）：指鉴定得到的蛋白质的Mascot总离子评分。覆盖度（Coverage）：指单个鉴定到的蛋白质所匹配的唯一肽段数目。MiD：线粒体残体分布（mitosomal distribution）。若蛋白质在Optiprep密度梯度（Optiprep gradient）的第3、4组分中的分布（通过同位素相对和绝对定量标记（iTRAQ）比值测定）处于Cpn10与IscU之间的区间，且向两侧延伸至这两个标记物间距一半的范围内，则标记为“Y”；分布超出该区间的蛋白质则标记为“N”。使用TargetP与PsortII对贾第虫（Giardia）蛋白质的亚细胞定位进行预测，其中S代表分泌型（secretory）、N代表非分泌型（non-secretory）、M代表线粒体型（mitochondrial）、O代表其他型（other）。Exp. ver.：实验验证（Exp. ver.），即使用pONDRA表达载体对蛋白质定位开展实验验证：通过荧光显微镜对带有标签的重组蛋白进行定位观察，其中M代表线粒体残体（mitosome）、ER代表内质网（endoplasmic reticulum）、O代表其他、?代表结果不确定。使用MEMSAT3与TMHMM对跨膜结构域进行预测。SGP：标记有井号（#）的为预测的可溶性蛋白。星号（*）用于表示无相关数据的条目。(**)代表转染的贾第虫未表达对应的带有标签的重组蛋白。