Gliding motility assay using 22063mM D2O in solution. Replicate 2/3. ROI 6/15.

• This is a gliding motility assay using kinesin-1 and rhodamine labeled microtubules.• The buffer used was PEM (also known as BRB80) with the inclusion of 22063mM D2O.• Images were taken with an Andor Luca EMCCD camera on an Olympus IX-71 microscope using a 60x 1.42NA PlanApo objective.• This fileset is replicate 2/3 and ROI 6/15.• Images were taken to investigate speed variations of microtubules due to the inclusion of D2O in the motility assay.• The temperature of the slide was held constant at 32°C by an objective heater. Early ROIs, i.e. 1-5, have slower giding speeds due to the increasing temperature of the slide which, was prepared at room temperature before observation. After ROI 5 the gliding speed stabilizes.• Pixels lengths were measured to be 166nm/pixel using a stage micrometer.• After the files have downloaded, a simple command line entry will convert the images to a movie. Mencoder must be installed. ls > list.txt | mencoder mf://@list.txt -mf w=496:h=658:fps=30:type=png -ovc lavc -lavcopts vcodec=mpeg4:mbd=2:trell -o output.avi• Upload of these files were made possible through the figshare API.• 本数据集为基于驱动蛋白-1（kinesin-1）与罗丹明标记微管的滑行运动检测实验。 • 实验所用缓冲液为PEM（又称BRB80），其中添加了22063mM 重水（D₂O）。 • 实验图像通过搭载Andor Luca EMCCD相机的Olympus IX-71显微镜拍摄，采用60×1.42NA PlanApo物镜。 • 本文件集对应第2/3组重复实验，以及感兴趣区域（Region of Interest，ROI）6/15。 • 本次成像实验旨在探究滑行运动检测体系中，因添加重水所引发的微管滑行速度变化情况。 • 通过物镜加热器将载玻片温度恒定维持在32℃。早期ROI（即1-5号）的滑行速度较慢，这是由于载玻片于观测前在室温下制备，随观测进程推移，载玻片温度逐渐升高；待观测至第5号ROI后，滑行速度趋于稳定。 • 采用载物台测微尺（stage micrometer）校准后，测得像素长度为166nm/像素。 • 图像文件下载完成后，可通过简单命令行指令将其转换为视频。需提前安装Mencoder软件，具体执行指令如下：ls > list.txt | mencoder mf://@list.txt -mf w=496:h=658:fps=30:type=png -ovc lavc -lavcopts vcodec=mpeg4:mbd=2:trell -o output.avi • 本数据集文件可通过figshare API完成上传。