Transcriptome analysis of medaka germ cells and gonadal somatic cells during the gonadal sex differentiation.. Oryzias latipes

Microarray analysis was performed using germ cells and gonadal somatic cells isolated by Fluorescence Activated Cell Sorter (FACS) from XX and XY embryos at st.33 and st.35. Overall design: Medaka germ cells (olvas-EGFP) and somatic cells (sox9b-DsRed) were isolated by FACS. The microarray experiments were done using three independent biological replicates.

本研究以第33期（st.33）和第35期（st.35）的XX、XY型青鳉胚胎为材料，通过荧光激活细胞分选术（Fluorescence Activated Cell Sorter, FACS）分离其生殖细胞与性腺体细胞，进而开展微阵列分析。实验整体设计如下：以olvas-EGFP标记的青鳉生殖细胞与sox9b-DsRed标记的体细胞为分选对象，经FACS完成分离；微阵列实验设置3次独立生物学重复。