Transcriptional changes in mycorrhizal and non-mycorrhizal soybean roots in presence of the fungal pathogen Macrophomina phaseolina. Glycine max

Macrophomina phaseolina, a soil borne pathogen with a wide host range, causes the charcoal rot in soybean (Glycine max (L.) Merr). In Argentina, soybean is the most important crop as far as sowing surface cultivation concerns, and for this reason it is important to assess integrated disease management strategies in order to minimize the incidence of this disease and the consequent loss of performance culture. Previous studies have demonstrated the protective effect that arbuscular mycorrhizal can promote on their hosts against various pathogens invasion, especially against soil borne fungi. Therefore, the goal of this study was to analyze the phenomenon of mycorrhizae mediated protection by characterizing transcriptional changes using cDNA microarrays as a tool. The objective of the present study was to investigate, under strict in vitro culture conditions, the global transcriptional change in the roots of pre-mycorrhized soybean plantlets challenged by M. phaseolina as compared to non-mycorrhizal soybean plantlets. The MapMan software was used to distinguish transcriptional change under these conditions, with special emphasis on plant defence response. Overall design: Four-condition experiment. Biological replicates: 3 replicates per condition. Roots of two plantlets were randomly selected and pooled for analysis (i.e. three biological replicates)

菜豆壳球孢菌（Macrophomina phaseolina）是一种寄主范围广泛的土传病原菌，可引发大豆（Glycine max (L.) Merr）的炭腐病。在阿根廷，大豆是播种面积居首的核心农作物，因此评估综合病害管理策略以降低该病害的发病率，并减少由此造成的作物生产性能损失具有重要意义。既往研究表明，丛枝菌根（arbuscular mycorrhizal）可对寄主产生保护效应，抵御多种病原菌侵染，尤其是土传真菌。据此，本研究旨在以cDNA微阵列（cDNA microarray）为工具，通过解析转录组变化来阐释菌根介导的保护作用机制。本研究的具体目标为：在严格的体外培养条件下，对比预接种丛枝菌根的大豆幼苗与未接种丛枝菌根的大豆幼苗在受到菜豆壳球孢菌侵染后的根部全局转录组变化。本研究采用MapMan软件分析上述条件下的转录差异，重点关注植物防御响应通路。实验整体设计：四处理组实验。生物学重复：每个处理组设置3次生物学重复，每次重复随机选取2株幼苗的根部混合后用于分析。