CIL:34929

Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell interior at cell edge (CIL 34926) of cytochalasin D-treated human 356 fibroblasts stained with ABP-280 primary antibody and 10-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal ABP-280 at filament crossovers in the cell interior. A fluorescence image of fibroblast lamellipodia double stained with TRITC-phalloidin (red) and ABP-280 (green) can be found at (CIL 34923). Image corresponds to Figure 5h from J Cell Biol. 1999 May 31;145(5):1009-26.

成纤维细胞细胞骨架中交联蛋白的定位研究。本图像为经细胞松弛素D（cytochalasin D）处理的人356成纤维细胞的细胞边缘区域（CIL 34926）的细胞内部免疫电子显微镜（Immuno-EM）成像：样本采用ABP-280一抗与10nm金偶联二抗染色。黄色金颗粒标记显示ABP-280定位于细胞内部的微丝交联位点。采用四甲基罗丹明异硫氰酸酯-鬼笔环肽（TRITC-phalloidin，红色）与ABP-280（绿色）进行双重染色的成纤维细胞片状伪足免疫荧光成像可参见CIL 34923。本图像对应《Journal of Cell Biology》1999年5月31日刊第145卷第5期第1009-1026页的图5h。