Data from the manuscript "Assessing the breeding phenology of a threatened frog species using eDNA and automatic acoustic monitoring"

- "data_acoustic.csv": hourly acoustic data (overall maximum number of calling male chorus frogs and number of calling male chorus frogs recorded by each songmeter A, B and C) - "IPC plate 1.ddpcr" and "IPC plate 2.ddPCR": Internal positive control raw data generated by the QX200 droplet digital PCR reader and associated software (QX Manager 1.2 Standard Edition)."Sample Description 1" column indicates the name used during fieldwork, "Sample Description 2" column indicates the sample name used in the manuscript (i.e. matching with the Figure 1 of the manuscript). - "sample plate 1.ddpcr", "sample plate 2.ddpcr","sample plate 3.ddpcr": raw data of 56 eDNA samples, positive and negative controls generated by the QX200 droplet digital PCR reader and associated software (QX Manager 1.2 Standard Edition). "Sample Description 1" column indicates the name used during fieldwork, "Sample Description 2" column indicates the sample name used in the manuscript (i.e. matching with the Figure 1 of the manuscript). - ".ab1" files are the cytb Sanger sequencing chromatograms and sequences of i) the 2021 water samples for assay validation (Bracken marsh locales 1 to 3; Cecil Graham Park locales 1 and 2, Old Mill Road locales 1 to 3, Round field marsh), ii) one <em>Pseudacris maculata</em> sample used as positive control (macPC), iii) two <em>Pseudacris triseriata </em>individuals (CF14 and CF56), iv) "12R4" is a positive 2022 eDNA sample (12L3 in the manuscript, see Figure 1).- "data_acoustic.csv"：每小时声学数据集，包含雄性合唱雨蛙的整体最大鸣叫数量，以及测声仪A、B、C分别记录到的鸣叫雄性合唱雨蛙数量。 - "IPC plate 1.ddpcr"与"IPC plate 2.ddPCR"：由QX200液滴数字PCR（droplet digital PCR, ddPCR）阅读器及其配套软件QX Manager 1.2标准版生成的内参阳性对照原始数据。其中"Sample Description 1"列标注野外采样阶段使用的样本名称，"Sample Description 2"列标注论文中使用的样本名称（即与论文图1的样本一一对应）。 - "sample plate 1.ddpcr"、"sample plate 2.ddpcr"与"sample plate 3.ddpcr"：由QX200液滴数字PCR阅读器及其配套软件QX Manager 1.2标准版生成的56份环境DNA（environmental DNA, eDNA）样本、阳性对照及阴性对照的原始数据。其中"Sample Description 1"列标注野外采样阶段使用的样本名称，"Sample Description 2"列标注论文中使用的样本名称（即与论文图1的样本一一对应）。 - ".ab1"格式文件为细胞色素b（cytochrome b, cytb）的Sanger测序色谱图及序列，涵盖以下四类样本：① 2021年用于检测验证的水样样本（包括布雷肯沼泽1~3号点位、塞西尔·格雷厄姆公园1~2号点位、老磨坊路1~3号点位以及朗德菲尔德沼泽点位）；② 1份用作阳性对照的斑腿雨蛙（*Pseudacris maculata*）样本（macPC）；③ 2份细纹雨蛙（*Pseudacris triseriata*）个体样本（CF14与CF56）；④ "12R4"为2022年阳性eDNA样本（论文中记为12L3，详见图1）。